Prostate cancer (PCa) is a difficult disease to understand. The tumor's genetic diversity increases as mutations accumulate, resulting in distinct subclones. On the other hand, non-genetic intra-tumoral heterogeneity, the cellular differentiation state, and the interaction between subclonal evolution and transcriptional heterogeneity, on the other hand, are poorly understood. The authors performed single-cell RNA sequencing on 14 untreated PCa patients in this study. They developed a comprehensive cell atlas of PCa patients and mapped developmental states onto tumor subclonal evolution. They find distinct subclones in PCa patients and then divide tumor cells into four transcriptional subtypes: EMT-like (subtype 0), luminal A-like (subtype 1), luminal B/C-like (subtype 2), and basal-like (subtype 3). These subtypes are organized hierarchically into stem cell-like and differentiated states. Surprisingly, multiple subclones within a single primary tumor have distinct preferential subtype combinations. Furthermore, subclones exhibit varying levels of communication with other cell types within the tumor ecosystem, which may modulate the distinct transcriptional subtypes of the subclones. Notably, they discovered that tumor cell transcriptional heterogeneity and cellular ecosystem diversity correlate with features of a poor prognosis by integrating TCGA data. Their research examines subclonal and transcriptional heterogeneity and its implications for patient prognosis.
Classification of Microarray ChipsJanuary 20, 2023A biochip or microarray chip is a microscope slide for the laboratory, usually made of silicon chip, glass, nylon film. It is usually a 2D array (sometimes 3D) with thousands of microwells at defined ...view
Introduction and Application of Microarray ChipJune 20, 20221. Definition of Microarray ChipMicroarray chip refers to the use of light-guided in-situ synthesis or micro-spotting methods to solidify a large number of biological macromolecules such as nucleic ac...view
Knowledge About Transcriptome SequencingDecember 1, 2022In a broad sense, the transcriptome refers to the collection of all transcripts in a cell under a certain physiological condition, including messenger RNA, ribosomal RNA, transfer RNA and non-coding R...view
Introduction of Cabin LaboratorySeptember 27, 20221. Definition of Cabin LaboratoryCabin laboratory is used for nucleic acid detection experiments, microorganism detection experiments, biochemical detection experiments, physical and chemical analysis...view