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16s 18s its

16s/18s/ITS

The full length of bacteria 16S rDNA is about 1540bp, including 10 conserved regions and 9 hypervariable regions, in which the difference of conserved region in bacteria is not significant while the hypervariable region has high specificity. Therefore, some hypervariable regions (usually V3 and V4 regions) can be sequenced to analyze the bacterial community diversity.


18S rRNA is commonly used in fungi for phylogenetics since it has more hypervariable domains than 16S. In addition to this, the ITS (Internal Transcribed Spacer) region, removed in the posttranscriptional process of nuclear rRNA cistron, has been widely regarded as a universal fungi barcode marker for a successful identification of the broadest range of fungi. And compared to 18S, ITS is more variable and hence more suitable as the genetic marker for measuring intraspecific genetic diversity.


Workflow Specifications

16s 18s its

Platform

Illumina NovaSeq6000

Read Length

PE250

Data Output

50K raw tags

Sample Requirements

DNA amount ≥150ng,DNA Concentration ≥5ng/μL

Integrity: The volume shall greater than 30μL and DNA shall have obvious main band without impurities contamination such as degradation, RNA and protein

Purity: OD260/280: 1.8~2.0, no impurities contamination such as RNA and protein

Standard Data Analysis

Data split and reads merging, Data quality control: data filtration and chimera removal, OTUs(Operational Taxonomic Units) Clustering and Species annotation,Species distribution, Alpha-diversity Analysis, Beta-diversity Analysis



Advantages of 16S/18S/ITS Amplicon Sequencing


 CapitalBio’s 16S/18S/ITS amplicon sequencing services offer several advantages for microbial analysis:


  • Common Genetic Markers: Our 16S/18S/ITS amplicon sequencing targets the most common housekeeping genetic markers with conserved and variable regions. This ensures comprehensive coverage and accurate representation of microbial diversity.


  • Fast Speed: With our advanced technology and streamlined processes, we provide fast turnaround times for results. Researchers can obtain valuable insights quickly, enabling timely decision-making.


  • Cost-Efficiency: We understand the importance of cost-effectiveness in research. Our 16S/18S/ITS amplicon sequencing services offer competitive pricing without compromising on quality, making it accessible to a wide range of researchers.


  • High Precision: Accuracy is paramount in microbial analysis. Capitalbio’s 16S/18S/ITS amplicon sequencing delivers high precision, ensuring reliable and reproducible results for confident interpretation and conclusions.


  • Multiple Applications: Our services have diverse applications, including microbial identification, diversity analysis, taxonomy and phylogeny, new species determination, and studying the relationship between microorganisms and diseases. Additionally, we support metagenomics research, enabling the analysis of complex microbial communities.


 CapitalBio is a trusted name in the industry, known for our expertise, advanced technology, and commitment to delivering comprehensive and efficient microbial analysis solutions.


What Is The Difference Between 16s,18s, And ITS Sequencing?


The 16S, 18S, and ITS sequencing methods are all used to study microbial diversity, but they target different types of organisms and offer varying levels of taxonomic resolution.


16S rRNA sequencing is primarily used in prokaryotic studies, as the 16S rRNA is a component of the 30S subunit in prokaryotic ribosomes. This method is highly effective for identifying and comparing bacteria present in a given sample.

18S rRNA sequencing targets the 18S rRNA, a component of the 40S subunit in eukaryotic ribosomes. This method is used to study the diversity of eukaryotic microbes, providing a broad spectrum of species annotations, albeit with slightly reduced accuracy due to the high conservation of the 18S rRNA gene.

ITS sequencing, meanwhile, targets the Internal Transcribed Spacer (ITS) regions, which are highly variable. This makes ITS sequencing particularly suitable for research on fungal diversity, as it allows for a high degree of accuracy in species annotations, even among closely related species.


The choice between 16S, 18S, and ITS sequencing offered by CapitalBio depends on the specific research requirements, whether it’s studying bacterial diversity, eukaryotic microbial diversity, or fungal species.


What Is The Importance Of 16s And 18s rRNA?


The 16S and 18S rRNA genes are highly conserved, meaning they remain relatively unchanged across different species. This makes them ideal markers for identifying and classifying different species. By sequencing these genes, researchers can determine the presence and relative abundance of different species in a sample. This is often referred to as the structure of the microbiome.


The 16S rRNA gene is primarily found in bacteria and archaea. By sequencing this gene, researchers can identify and compare bacterial species in a given sample. This is particularly important in fields like health and disease research, where understanding the bacterial composition of the human microbiome can have significant implications.


The 18S rRNA gene is found in eukaryotes. Sequencing this gene allows researchers to study the diversity of eukaryotic microbes. This is crucial in environmental science, for example, where understanding the microbial composition of soil or water samples can inform conservation efforts.


CapitalBio’s sequencing services provide the necessary tools and expertise to accurately sequence these rRNA genes, providing valuable insights into the structure and diversity of microbial communities.


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