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mrna sequencing

mRNA Sequencing CapitalBio

Transcriptome research is the basis and starting point of gene function and structure research. Through a new generation of high-throughput sequencing technology, we can comprehensively and quickly obtain the information expressed by mRNA of a specific tissue or organ in a certain state. At present, it has been widely used for transcript structure (gene boundary identification, alternative splicing research, etc.), transcript variation research (gene fusion, coding region SNP research, etc.), gene expression level research and new transcript discovery.

Biomarker Discovery
Drug Development
Evolutionary Study
Resistance And Adaptability Study
Workflow Specifications Data Analysis(Demo Results Sample)




Read Length


Data Output

Eukaryotes: 6G; Prokaryotes: 1/2G

Sample Requirements

RNA amount > 400 ng

RIN > 7

Purity: A260/280 = 1.8-2.2, A260/230 ≥ 1.8

Data Analysis

  • Data QC

  • Gene Expression Quantification

  • Differential Expression Profiling

  • Functional Enrichment Analysis

  • Novel Transcripts Identification

  • SNP and InDel Analysis

  • Alternative Splicing Analysis

  • Fusion Gene Prediction

  • Protein-Protein Interaction Analysis

  • Transcription Factors and Oncogene Functional Annotation


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