Transcriptome research is the basis and starting point of gene function and structure research. Through a new generation of high-throughput sequencing technology, we can comprehensively and quickly obtain the information expressed by mRNA of a specific tissue or organ in a certain state. At present, it has been widely used for transcript structure (gene boundary identification, alternative splicing research, etc.), transcript variation research (gene fusion, coding region SNP research, etc.), gene expression level research and new transcript discovery.