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lncrna

lncRNA Sequencing CapitalBio

Long non-coding RNA (lncRNA) is is a kind of non-coding protein RNA with length more than 200nt. It regulates gene expression by binding with DNA, RNA or protein. Illumina platform was used for sequencing, lncRNA analysis was carried out for reference genome species, and the analysis of mRNA in sequencing data was also provided.


Profiling Known And Novel Transcripts And Identifies Variations
Predicting Targeting Genes Of Lncrna
Identifying Biomarkers For Cancer/disease Diagnostics And Classification
Discovering Regulation Between lncRNA And mRNA
Workflow Specifications

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Platform

NovaSeq6000

Read Length

PE150

Data Output

12G

Sample Requirement

RNA amount > 500ng

RIN > 7

Purity: A260/280 = 1.8-2.2, A260/230 ≥ 1.8

Standard Data Analysis

  • Data QC

  • Structural Analysis

  • lncRNA Identification and Annotation

  • Expression Quantification and Differential Expression Profiling

  • Functional Enrichment Analysis

  • Protein-Protein Interaction analysis

  • lncRNA Target Gene Prediction



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