lncRNA Sequencing

Long non-coding RNA (lncRNA) is is a kind of non-coding protein RNA with length more than 200nt. It regulates gene expression by binding with DNA, RNA or protein. Illumina platform was used for sequencing, lncRNA analysis was carried out for reference genome species, and the analysis of mRNA in sequencing data was also provided.

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Profiling Known And Novel Transcripts And Identifies Variations

Predicting Targeting Genes Of Lncrna

Identifying Biomarkers For Cancer/disease Diagnostics And Classification

Discovering Regulation Between lncRNA And mRNA

Workflow Specifications

Platform	NovaSeq6000
Read Length	PE150
Data Output	12G
Sample Requirement	RNA amount > 500ng
	RIN > 7
	Purity: A260/280 = 1.8-2.2, A260/230 ≥ 1.8
Standard Data Analysis	Data QC Structural Analysis lncRNA Identification and Annotation Expression Quantification and Differential Expression Profiling Functional Enrichment Analysis Protein-Protein Interaction analysis lncRNA Target Gene Prediction

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