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lncrna

lncRNA Sequencing CapitalBiotech

Long non-coding RNAs (lncRNAs) are non-protein-coding RNA transcripts longer than 200 nucleotides. They can regulate gene expression through interactions with DNA, RNA, and proteins. Sequencing is performed on the Illumina platform for species with a reference genome, with integrated analysis of both lncRNA and mRNA expression.

Profiling Known and Novel Transcripts and Identifying Variants
Predicting Target Genes of lncRNAs
Identifying Biomarkers for Cancer and Disease Diagnosis and Classification
Investigating Regulatory Relationships Between lncRNAs and mRNAs
Workflow Specifications Standard Data Analysis

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Platform

NovaSeq 6000

Read length

PE150

Data output

12 Gb

Sample Requirement

RNA amount: >500 ng

RIN > 7

Purity: A260/A280 = 1.8–2.2; A260/A230 ≥ 1.8

Standard Data Analysis

  • Data QC

  • Structural Analysis

  • lncRNA Identification and Annotation

  • Expression Quantification and Differential Expression Profiling

  • Functional Enrichment Analysis

  • Protein-Protein Interaction Analysis

  • lncRNA Target Gene Prediction



Data Quality Control; 

Transcript Structure Analysis; 

lncRNA Identification and Annotation; 

Expression Quantification and Differential Expression Analysis; 

Functional Enrichment Analysis; 

Protein–Protein Interaction Analysis;

lncRNA Target-Gene Prediction

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