Microarray chip refers to the use of light-guided in-situ synthesis or micro-spotting methods to solidify a large number of biological macromolecules such as nucleic acid fragments, polypeptide molecules and even biological samples such as tissue sections and cells on supports (such as glass slides, nylon membranes) in an orderly manner. The surface of the carrier) is composed of dense two-dimensional molecular arrangements, and then reacts with the target molecules in the labeled biological sample to be tested, and the intensity of the response signal is measured by specific instruments, such as a laser confocal scanner or a charge-coupled camera. Perform fast, parallel, and efficient detection and analysis to determine the number of target molecules in a sample.
2. Microarray chip application process
(1) Preparation of targets
Extract and label nucleotides from biological specimens;
(2) Hybridization
Incubate the target with the cDNA or oligonucleotide sequence on the chip;
(3) Get data
Scan the signal intensity exhibited by the target hybridized to the probe
(4) Data analysis
Draw biologically meaningful conclusions from large amounts of data
Microarray chip technology reflects the transcription of the gene expressing this mRNA by measuring the number of mRNA that can hybridize with the probe. The construction of the chip should first select the gene and the corresponding probe according to the needs of the research, and then extract the mRNA from the sample. , and prepare the target point, then add the target point to the chip, carry out the operations of incubation hybridization, washing out the unhybridized samples and scanning, etc., to obtain the original data, and then standardize and statistically analyze these data to get the conclusion, and construct an appropriate microarray. The array chip is the basis for the follow-up research.
Application Prospects of in Vitro Diagnostic TechnologyMarch 9, 2023The development of in vitro diagnostic (IVD) technologies has been rapid, spanning from genetic sequencing, SNP screening, point mutation gene diagnosis at the gene level, to the detection of various ...view
Talking about Gene SequencerJune 20, 20221. Definition of Gene SequencerGene sequencer, also known as DNA sequencer, is an instrument for determining the base sequence, type and quantification of DNA fragments. It is mainly used in human gen...view
One Of The Most Common Birth Defects In The World Is Hearing LossJune 28, 2022One of the most common birth defects in the world is hearing loss. Every 1,000 babies are born with permanent hearing loss in one or both ears. Hearing loss can have a significant impact on a baby'...view
Microarray Chip Application Process and Data AnalysisDecember 15, 2022Microarray chip refers to the use of photoconductive in-situ synthesis or micro spotting to solidify a large number of biological macromolecules such as nucleic acid fragments, polypeptide molecules, ...view
Technical Principles and Functions of Gene SequencingDecember 1, 2022Gene sequencing is a new type of genetic testing technology, which can analyze and determine the full sequence of genes from blood or saliva, predict the possibility of suffering from various diseases...view
Basic Performance of In Vitro Diagnostic Reagent ProductsMarch 9, 20231. Blank IVD in vitro diagnostic reagentsThe reagent blank can be understood as the value detected when the content of the substance to be tested is the theoretical zero value. The general practice is...view