Whole Genome Re-sequencing

All DNA sequences of certain individual genome can be determined in one time through genomic sequencing, i.e. WGS (whole genome re-sequencing). Detect the variation on the genome level, including SNP, InDel, SV and CNV, by combining the analysis on biological information. As a powerful research tool, genomic sequencing is widely applied in scientific and clinical research, and its huge sequencing data provide comprehensive basis for analyzing the interindividual difference, population evolution and clinical disease research.

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Application of Animal/Plant WGS

Application of Human WGS

Research On The Origin And Evolutionary History Of Species

Research on population queue and evolution: Normal population queue, Disease population queue, Cumulative mutation of environmental factors

Molecular Marker Development, Assisted Quality Improvement And Variety Breeding

Research on genetic disease: Monogenic disease, Rare disease, Complex disease, De novo mutation

Functional Gene Mining For Target Traits And Gene Mapping For Excellent Traits

Research on cancer genome: Tumor susceptibility gene, Pathogenesis of tumors, Tumor metastasis/relapse/heterogeneity

Trait Inheritance Mechanism Analysis

Research on pharmacogenomics: Drug-resistance mechanism, Targeted drag therapy

Assisted Germplasm Innovation And Identification

Workflow Specifications Demo Results

human whole genome sequencing

	Human WGS	Animal/Plant WGS
Platform	NovaSeq6000
Read Length	PE150
Recommended Sequencing Depth	For genetic disease: 30-50X For tumor: 50X	For SNP/InDel detection: ≥ 10× For SV/CNV detection: ≥ 20×

Sample Requirements	Purity: OD260/280 is within the range of 1.7-2.1
	DNA amount ≥3μg
	DNA Concentration ≥20ng/μL
	Integrity:main band of genome is clear and free from RNA contamination, protein contamination or degradation, with molecular weight ≥10Kb.
Data Analysis	★Raw data quality control ★Alignment with reference genome ★SNP/InDel/SV/CNV calling ★More data mining upon your request

Figure Type	Figure
Circos Plot
Heat map of Differential Gene Cluster
Heat Map of High-frequency Mutant Gene Cluster
Example Diagram of Path Bubble Ranked Top 30 of q-value in KEGG PATHWAY
Diagram of GO Functional Enrichment Layer
Diagram of Protein Interaction Network

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