CapitalBiotech® Microarray Hybridization Station

Features of CapitalBiotech® Microarray Hybridization Station

1. When paired with CapitalBiotech's specially designed hybridization cassettes, it achieves uniform temperature and humidity control.

   
   

2. Unique 3-D motion technology ensures homogeneous hybridization signals across the entire microarray surface.

   
   

3. Eliminates edge effects: Enhanced performance is validated in recent independent studies on microarray quality, such as Patterson, T., et al., (2006). Nature Biotechnology, 24: 1140-1150, and Shi, L., et al., (2006). Nature Biotechnology, 24: 1151-1161.

   
   

4. Equipped with an LCD control panel for intuitive operation.

   
   

5. Compatible with microarray hybridizations and immunoreactions on 25 mm × 75 mm slides, or in tubes using different adapters.

What Is The Difference Between Microarray Hybridization And Northern Hybridization?

Northern hybridization, also known as Northern blotting, is a technique used to detect specific RNA molecules in a mixture. In this process, a single DNA probe is labeled and hybridized with an unlabeled RNA mixture. This enables the detection and quantification of specific RNA molecules, making it a targeted approach.

Microarray hybridization is a high-throughput method that allows for the simultaneous analysis of thousands of genes. In this technique, a mixture of target RNA, which may consist of multiple species of molecules, is labeled. This labeled mixture is then hybridized to a solid surface containing thousands of immobilized probes, each corresponding to a specific gene. This allows for the simultaneous detection and quantification of numerous different RNA molecules, providing a comprehensive analysis of gene expression.

The main difference between the two techniques lies in their scale and specificity: while Northern hybridization focuses on a single RNA molecule, microarray hybridization allows for a broader, more comprehensive analysis. Both techniques have their advantages and are used in different contexts based on research needs.

What Is The Principle Of Microarray Hybridization DNA Chip Technology?

Microarray hybridization is a technique based on the principle of complementary base pairing. In this process, DNA strands from a sample are labeled and applied to a DNA microarray, where they hybridize to their complementary sequences. These sequences are pre-immobilized at specific spots on the microarray surface. The hybridization process, is facilitated by a microarray hybridization station, which maintains optimal conditions to ensure specific and efficient binding.

The use of a microarray hybridization station ensures high accuracy and efficiency in the hybridization process. This enables the simultaneous analysis of thousands of genes, providing comprehensive insights into gene expression patterns and genetic variations. CapitalBiotech deliver this technology to deliver precise and reliable genomic analyses.

How Is Hybridization Used In Microarray?

• Microarray hybridization involves allowing two samples of cDNA, labeled with different fluorescent dyes, are allowed to hybridize to a microarray slide.

• The slide contains a grid of DNA probes, each corresponding to a specific gene.

• Hybridization occurs when these cDNA molecules (bind or ‘hybridize’) to complementary DNA probes on the slide; this binding is highly sequence-specific.

• After hybridization, the slide is transferred to a microarray hybridization station provided by CapitalBiotech. This device maintains optimal conditions, ensuring efficient and accurate hybridization.

• Controlling the hybridization environment with such a station is critical, as environmental factors significantly impact the results.

• The microarray is then scanned to measure fluorescence intensity at each spot. The intensity of the fluorescence indicates the expression level of the corresponding gene.

• By comparing fluorescence signals from the two dyes, researchers identify differences in gene expression between samples.

• CapitalBiotech’s microarray hybridization station serves as a key tool in this process, providing reliable and efficient solutions for researchers worldwide.

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