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metagenomics shotgun sequencing

Shotgun Metagenomics Sequencing

Metagenome refers to the sum of all microbial genetic materials in specified environment. Metagenome sequencing can determine all microorganisms in specified environment, especially conduct sequence determine and excavate functional gene for the genome which is difficult to culture the microorganism and research new gene with specified function.

Workflow Specifications Bioinformatics Analysis

shotgun metagenomics

PlatformNovaSeq 6000
Read lengthPaired-end 150bp
Sequencing Depth


Sample RequirementsPurity: OD260/280: 1.8~2.0, no impurities contamination such as RNA and protein

DNA amount ≥1.6μg

DNA Concentration ≥50ng/μL

Integrity:The volume shall greater than 30μL and DNA shall have obvious main band without impurities contamination such as degradation, RNA and protein


Metagenome Sequencing

16S rDNA Segment Sequencing

Test Area

Test the whole genomic DNA sequence

Test the 16S hypervariable region and usually as V3+V4

Identified Depth

Species level

Genus level

Research Purpose

Except achieving the 16S rDNA research purpose, functional genome analysis, inter-specimen gene difference, metabolic network of researched species can also be conducted

Research the microflora structure, abundance, diversity, evolution relationship and microflora difference etc.

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