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metagenomics shotgun sequencing

Shotgun Metagenomics Sequencing

Metagenome refers to the sum of all microbial genetic materials in specified environment. Metagenome sequencing can determine all microorganisms in specified environment, especially conduct sequence determine and excavate functional gene for the genome which is difficult to culture the microorganism and research new gene with specified function.


Workflow Specifications Bioinformatics Analysis

shotgun metagenomics

PlatformNovaSeq 6000
Read lengthPaired-end 150bp
Sequencing Depth

6G/12G

Sample RequirementsPurity: OD260/280: 1.8~2.0, no impurities contamination such as RNA and protein

DNA amount ≥1.6μg

DNA Concentration ≥50ng/μL

Integrity:The volume shall greater than 30μL and DNA shall have obvious main band without impurities contamination such as degradation, RNA and protein




Item

Metagenome Sequencing

16S rDNA Segment Sequencing

Test Area

Test the whole genomic DNA sequence

Test the 16S hypervariable region and usually as V3+V4

Identified Depth

Species level

Genus level

Research Purpose

Except achieving the 16S rDNA research purpose, functional genome analysis, inter-specimen gene difference, metabolic network of researched species can also be conducted

Research the microflora structure, abundance, diversity, evolution relationship and microflora difference etc.


How Is Metagenomics Shotgun Sequencing Different From 16s?

CapitalBio utilizes shotgun metagenomic sequencing, a technique that differs significantly from 16S rRNA sequencing. While 16S rRNA sequencing targets a specific region of DNA, shotgun metagenomic sequencing reads all genomic DNA present in a sample. This comprehensive approach allows for the identification and profiling of a wide range of microorganisms, including bacteria, fungi, and viruses. Therefore, for microbiome studies, shotgun sequencing provides a more complete and detailed picture of microbial diversity and function.


Advantages Of Shotgun Metagenomics Sequencing

CapitalBio employs shotgun metagenomic sequencing due to its numerous advantages. This method is faster as it eliminates the need for the mapping process, thus accelerating the sequencing timeline. Shotgun metagenomic sequencing also requires less DNA, making it more efficient and cost-effective, especially when dealing with scarce or precious samples. Furthermore, the cost of shotgun sequencing is significantly lower than other methods that require a map, making it a more economical choice. Overall, shotgun metagenomic sequencing offers a comprehensive, efficient, and cost-effective solution for studying microbial diversity and function.


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