Shotgun Metagenomics Sequencing

Metagenome refers to the sum of all microbial genetic materials in specified environment. Metagenome sequencing can determine all microorganisms in specified environment, especially conduct sequence determine and excavate functional gene for the genome which is difficult to culture the microorganism and research new gene with specified function.

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Workflow Specifications Bioinformatics Analysis

shotgun metagenomics

Platform	NovaSeq 6000
Read length	Paired-end 150bp
Sequencing Depth	6G/12G
Sample Requirements	Purity: OD260/280: 1.8~2.0, no impurities contamination such as RNA and protein
	DNA amount ≥1.6μg
	DNA Concentration ≥50ng/μL
	Integrity:The volume shall greater than 30μL and DNA shall have obvious main band without impurities contamination such as degradation, RNA and protein

Item	Metagenome Sequencing	16S rDNA Segment Sequencing
Test Area	Test the whole genomic DNA sequence	Test the 16S hypervariable region and usually as V3+V4
Identified Depth	Species level	Genus level
Research Purpose	Except achieving the 16S rDNA research purpose, functional genome analysis, inter-specimen gene difference, metabolic network of researched species can also be conducted	Research the microflora structure, abundance, diversity, evolution relationship and microflora difference etc.

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