SNP Detection CapitalBiotech

Variants identified at the genome-wide level often require independent validation in larger sample cohorts. The Agena MassARRAY® platform provides an efficient medium-throughput genotyping solution for targeted panels of fewer than 300 SNP loci.

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Medicine

Population Cohort Studies;

Disease Risk Assessment and Management.

Agriculture

Biomarker Validation;

Plant-Line Validation and Germplasm Identification;

Identification of Transgenic Organisms;

Marker-Assisted Selection;

Animal Genotyping

Experiment Process Specifications Advantages Agena Massarray Mass Spectrometry Platform

Experiment Process

Sample Requirements	Purity: OD260/OD280 = 1.7–2.1
	DNA amount: ≥1μg
	DNA concentration: ≥20 ng/µL
	Integrity: The main genomic DNA band should be clear, with no evident RNA or protein contamination or degradation; fragment size should be ≥10 kb.

Flexible	Select SNP loci and sample sets according to project requirements
Accurate	Direct mass-based detection supports accurate discrimination of alleles
High throughput	Up to 40-plex assays per reaction well, with 384-sample processing supported per chip
Cost-effective	Label-free detection helps reduce per-genotype analysis costs and is well suited to large-sample validation projects involving fewer than 300 SNP loci
Convenient	Assay design is available for more than 95% of validated SNPs, supported by dedicated primer-design and genotyping software for automated genotype calling and quality assessment