Small RNA Sequencing CapitalBiotech

Small RNAs are a diverse class of non-coding RNAs, typically 18–30 nucleotides in length, including miRNAs, piRNAs, endogenous siRNAs (endo-siRNAs), and snoRNAs. miRNAs, one of the most widely studied classes, are typically 20–24 nucleotides long and are found in both animals and plants. In animals, miRNAs commonly regulate gene expression by repressing translation and/or promoting target mRNA degradation. In plants, miRNAs frequently direct cleavage of target mRNAs. Sequencing is performed on the Illumina platform, with miRNA analysis available for species with a reference genome.

Get A Quote

Profiling Known and Novel Small RNAs and Quantifying Their Expression

Predicting Target Genes of Candidate miRNAs

Identifying Tissue-, Stage-, or Cell-Type-Specific Biomarkers for Cancer and Disease Diagnosis and Classification

Investigating Regulatory Networks and Developmental Mechanisms Based on Small-RNA Expression Profiles

Workflow Specifications Data Analysis

Workflow

Platform	NovaSeq 6000
Read length	SE50
Data output	>10 million reads
Sample Requirements	RNA amount: >2 µg
	RIN > 7
	Purity: A260/A280 = 1.8–2.2; A260/A230 ≥ 1.8
Data Analysis	Data QC Length Distribution Summary Common and Specific Sequence Summary Identification and Characterization of miRNAs Classification and Annotation of miRNA Quantification and Differential Expression Analysis Functional Enrichment Analysis