Single-Cell Sequencing

With the development of single-cell technology, the scope of research is no longer limited to the transcriptome, but expanded to the multi-omics level such as the genome, immunome, epigenome, and proteome. In complex biological processes, heterogeneity exists at multiple levels, cells with the same genes may have different patterns of DNA methylation, gene expression, and clonal expansion. To reveal deeper biological mechanisms, multi-omics techniques are required to more accurately classify them into different subgroups.