Microarray chip refers to the use of light-guided in-situ synthesis or micro-spotting methods to solidify a large number of biological macromolecules such as nucleic acid fragments, polypeptide molecules and even biological samples such as tissue sections and cells on supports (such as glass slides, nylon membranes) in an orderly manner. The surface of the carrier) is composed of dense two-dimensional molecular arrangements, and then reacts with the target molecules in the labeled biological sample to be tested, and the intensity of the response signal is measured by specific instruments, such as a laser confocal scanner or a charge-coupled camera. Perform fast, parallel, and efficient detection and analysis to determine the number of target molecules in a sample.
2. Microarray chip application process
(1) Preparation of targets
Extract and label nucleotides from biological specimens;
(2) Hybridization
Incubate the target with the cDNA or oligonucleotide sequence on the chip;
(3) Get data
Scan the signal intensity exhibited by the target hybridized to the probe
(4) Data analysis
Draw biologically meaningful conclusions from large amounts of data
Microarray chip technology reflects the transcription of the gene expressing this mRNA by measuring the number of mRNA that can hybridize with the probe. The construction of the chip should first select the gene and the corresponding probe according to the needs of the research, and then extract the mRNA from the sample. , and prepare the target point, then add the target point to the chip, carry out the operations of incubation hybridization, washing out the unhybridized samples and scanning, etc., to obtain the original data, and then standardize and statistically analyze these data to get the conclusion, and construct an appropriate microarray. The array chip is the basis for the follow-up research.
Decoding Genomic Medicine: The Power of Clinical WES in Patient CareJanuary 4, 2024In recent years, genomic medicine has revolutionized the way healthcare professionals diagnose and treat various diseases. A key component of this approach is Clinical WES (Whole Exome Sequencing), wh...view
The Beijing Winter Olympics Has Raised The Curtain On Feb 4, 2022May 19, 2022The Beijing Winter Olympics has raised the curtain on Feb 4, 2022, as thousands of athletes from across the globe gather in China for the first international multi-sport event to be held as scheduled ...view
What Are the Diagnostic Methods for Breast Cancer?April 4, 2023Breast cancer has become a terrifying disease that kills female friends with increasing clinical incidence rate. It not only harms women's health but also has a great impact on our normal life. So...view